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CPS1 Markerless Deletion Mutant as a Method for Marker Deletion

This technology involves a novel system using a two-step process to eliminate a gene of interest by incorporating a “suicide gene” vector system. The construct created by this method results with no foreign sequences, making the strains markerless mutants. This means that one has the potential to make genetic strains with multiple mutations...

Published: 4/23/2024 | Inventor(s): Maria Alejandra Mandel, Marc Orbach

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Category(s): Technology Classifications > Life Sciences, Technology Classifications > Research Tools > Screening assays

A Vector System for Direct Cloning of Eukaryotic Gene Constructs

Yeast-Agrobacterium-Bacteria (YAB) is a combination of two technologies: yeast recombination capabilities and Agrobacterium tumefaciens-mediated transformation (ATMT). Generation of ATMT constructs usually requires PCR-amplification of DNA fragments and multiple cloning steps into binary Agrobacterium vectors. YAB is a plasmid vector that can be used...

Published: 2/7/2024 | Inventor(s): Maria Alejandra Mandel

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Category(s): Technology Classifications > Research Tools, Technology Classifications > Research Tools > Cell Lines