Invention:
The inventors have combined an error-correcting information strategy for DNA with a supercritical fluid method for drying and storing DNA. The system features an apparatus suitable for end-to-end programmable DNA storage automation using a write-to-store-to-read data cycle via a programmable microfluidic platform (PMP). The system also features a method for solid-state preservation of DNA data on synthetic bone or a metal-organic nanoparticle matrix using the supercritical nitrogen drying (ScND) process. With these features, the system will be able to provide a tailored error correction-based DNA data storage device and a solid-state preservation of DNA data storage to overcome all the existing challenges of high-density data storage.
Background:
Synthetic DNA storage is a promising candidate with high density and long-term preservation for archival storage systems. If non-biological information is stored and distributed over prolonged periods of DNA, artificial protection is required because of degradation from hydrolysis, water, UV radiation, oxidation, ROS, ionizing radiation, heat, mutagenic chemicals or nucleases, and pH. Traditional DNA data storage methods have logistical constraints and significant expenses to maintain the appropriate conditions. Hence, effective DNA data storage systems require complete automation in the “write-to-store-to-read” cycle of data storage, high DNA data loading, increased DNA stability, and simple sample handling. Previous DNA data storage studies in solution have clearly shown that degradation rates can be precisely predicted, and buffer conditions can be adjusted to obtain remarkable stability at room temperature. Consequently, several approaches to storing digital information on DNA have been proposed with the error-correction model. However, those approaches are unreliable as they cannot handle errors efficiently and do not suggest how to (physically) store the DNA to maintain its stability over time.
Applications:
- Error-correcting information strategy
- Solid-state preservation of DNA data
- DNA data storage system
Advantages:
- Store DNA for extended periods of time
- Maintain DNA’s stability over time
