Invention:
Researchers at the University of Arizona have developed a novel microscope imaging technique that generates high-resolution large-volume 3D images of tissue at subcellular resolution, and captures transient activities within the volume at 100 volume frames per second (vps). The invention breaks away from the traditional plane-scanning approach and implements volumetric projection imaging instead.
Background:
In order to study complex dynamics of tissue in live animals, ideally the microscope needs to maintain the sub-micron resolution in deep tissue to resolve activities in subcellular structures, cover a large volume to analyze complex networks, and refresh the volumetric image at high speed to capture transient dynamics. However, despite many processes, at present there are no known microscopic techniques that fully satisfy the need for resolution, penetration, volume and speed.
Applications:
- Fast 3D subcellular imaging for organs, tissues, and other body parts
Advantages:
- Intrinsic high 3D resolution
- Simplified image processing
- Faster frame rates; can accommodate movement in sample
- Large image area/field of view
- Twice the photon sensitivity for increased photon efficiency
