Invention:
This invention presents a novel method and optical design for microscopic imaging that achieves high resolution, wide field of view (FoV), and large imaging depth without requiring mechanical scanning elements. This involves leveraging a MEMS-based phase light modulator (PLM) device into the imaging path so that the system expands both spatial and axial dimensions, enabling dense imagery acquisition over a significantly larger probe volume. The modular design with a compact form factor allows for seamless integration with existing microscope systems, offering enhanced imaging capabilities across a wide array of applications.
Background:
Currently, microscopy techniques face trade-offs between resolution, FoV, and depth of measurement. Cellular resolution is typically limited to small, shallow imaging regions, which require time-consuming and complex mechanical scanning for comprehensive 3D visualization. 3D images at cellular resolution are crucial in the biomedical imaging field and in semiconductor manufacturing. This invention could lay the foundation for high-throughput microscopy devices and will provide a scalable, efficient solution for next-generation microscopy applications, from whole brain imaging of freely behaving zebrafish to semiconductor wafer inspection.
Applications:
- Optical imaging
- Microscopy
- 3D imaging at cellular resolution
- Biomedical research
Advantages:
- Higher microscopy resolution
- Wider microscopy field of view
- Larger microscopy measurement depth
- Versatility in scientific and commercial applications
- Acquisition of dense imagery over a large probe volume
