Invention:
This technology applies to single cell RNA sequencing data that is created in parallel (multiplexed) to speed the process. Once the multiplexed data exists, it needs to be recombined into one set (demultiplexed) to be useful. This technology is a new demultiplexing technique that uses dyes combined with DNA barcodes, simplifying the demultiplexing process.
Background:
RNA sequencing (RNA-seq) is a next-generation sequencing technique that is used to detect and analyze RNA molecules to understand how a cell works. Compared to older techniques, it is faster, more reliable, and more cost-effective. The technique is useful in a variety of applications, including research into gene expression, discovery of new genes, and research into diseases, especially cancer.
State-of-the-art RNA-seq technology enables high throughput analysis by multiplexing techniques. In the end, the information needs to be recombined. There are a variety of existing demultiplexing techniques, but this one further streamlines existing techniques, making use of dyes combined with DNA barcodes to demultiplex the information at a reduced cost and without the need for as many reagents as existing techniques.
Applications:
- Single-cell RNA sequencing
- Genomics
- Biomedical research
- Cancer research
Advantages:
- Decreased cost
- Uses less reagents
- Simplified process
