Invention:
Researchers at the University of Arizona investigated the antimicrobial properties of Phenyllactic Acid (PLA) derivatives against both commensal and Bacterial vaginosis (BV)-associated bacteria, as well as their cytotoxic effects on human cervical epithelial cells. PLA is a broad-spectrum antimicrobial compound produced by vaginal Lactobacillus species; however, its mechanisms and functions within the cervicovaginal microenvironment are not well understood. To address the solubility issue of PLA, the researchers synthesized PLA ionic liquids (ILs) using choline and carnitine counterions, which allowed for the solubilization of D- and L-enantiomers of PLA in water. Various assays were conducted to evaluate the antimicrobial activity of the PLA ILs against different bacterial species, and it was found that antimicrobial activity varied depending on the stereochemistry of PLA and the counterion present in the ILs, but was effective overall. Overall, this study highlights the potential of PLA and its derivatives as antimicrobial agents against bacterial vaginosis. The stereochemistry of PLA and the choice of counterion in the ILs play a significant role in their antimicrobial activity, while the counterion affects the cytotoxicity to human cervical cells. Further investigations are underway to determine optimal inhibitory concentrations and explore additional formulations of PLA for potential therapeutic applications in the cervicovaginal microenvironment, which could lead to the development of more effective, targeted treatments for BV.
Background:
Bacterial vaginosis (BV) is a condition characterized by lactobacilli depletion and overgrowth of anaerobic bacteria in the vagina and cervix, which can lead to various adverse gynecologic and obstetric outcomes. Current technologies for treating BV, such as antibiotics, often have limitations and may lead to the development of antibiotic resistance. In addition, the solubility of Phenyllactic Acid (PLA), a known antimicrobial metabolite produced by vaginal Lactobacillus species, poses a challenge for its effective use.
Applications:
- Treatment of Bacterial Vaginosis (BV)
Advantages:
- Improved antimicrobial activity
- Soluble solution
- Optimized effectiveness in varying formulations of PLA
